Root exudates control critical processes in the rhizosphere, retaining water, selecting for beneficial microorganisms or solubilising nutrients prior to uptake by the plant. Analysing root exudation patterns however is challenging because existing methods are often destructive and unable to resolve spatial and temporal variations in the production of root exudates. Here, we present a paper-based microfluidic device with integrated colorimetric sensors for the continuous extraction and analysis of root exudates along plant roots. The microfluidic device used standard filter paper wax printer to create channels for water to carry the exudates towards the sensors. TiO2 nanotubes/alginate hydrogel-based sensors were used to analyse the glucose content of the root exudates of living plants. The study shows that the paper microfluidic substrate successfully extracts the released glucose from the root, and transfers it to the hydrogel-based sensor to be colorimetrically detected from independent sections of the root at different times, up to 7 days. This method was tested on two different wheat varieties Triticum aestivum (rgt Tocayo and Filon varieties), where significant differences in exudation patterns were recorded. This research demonstrates the feasibility of low-cost technological solution for high precision screening and diagnostic of the biochemical composition of root exudates.
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Agricultural run-off and subsurface drainage tiles transport a significant amount of nitrogen and phosphorus leached after fertilization. alchemia-nova GmbH in collaboration with University of Natural Resources and Life Sciences, Vienna developed two multi-layer vertical filter systems to address the agricultural run-off issue, which has been installed on the slope of an agricultural field in Mistelbach, Austria. While another multi-layer addressing subsurface drainage water is implemented in Gleisdorf, Austria. The goal is to develop a drainage filter system to retain water and nutrients. Both multi-layer filter systems contain biochar and other substrates with adsorption properties of nutrients (nitrogen, phosphorus). The filter system can be of practical use if an excess of nutrients being washed out is of concern in the fields of the practitioner by keeping the surrounding waters clean. This approach may result in economic value by re-using the saturated biochar as fertilizer and improving the soil structure, thus increasing long-term soil fertility. Link: https://wateragri.eu/a-bio-inspired-multilayer-drainage-system/
This project has received funding from the European Union’s Horizon 2020 research and innovation program under Grant Agreement No 858735This project has received funding from the European Union’s Horizon 2020 research and innovation program under Grant Agreement No 858735. FACTSHEET NANOCELLULOSE MEMBRANES FOR NUTRIENT RECOVERY Key information Functionalized nanocellulose membranes can take up nitrate and phosphate. These membranes can be put in a water treatment unit. As the membranes are biobased, degradable materials, they can after use be added to the soil, thus returning the leached nutrients back for their original purpose providing fertilizers (nutrient recycling).
Because variables such as temperature and humidity have a profound effect on the activity of crop pests, diseases and natural enemies, the ability to monitor environmental conditions within a crop has always been important for crop protection.