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    • Crop farming

    Metschnikowia pulcherrima as a Biocontrol Agent against Potato (Solanum tuberosum ) Pathogens

    An increasing trend in protecting plants against phytopathogens is the replacement of chemical pesticides with environmentally acceptable biopreparations. This article focuses on the possible use of yeast Metschnikowia pulcherrima as a biocontrol agent against potato pathogens. The scope included an assessment of the antimicrobial activity of 10 M. pulcherrima isolates against 10 phytopathogens: Fusarium oxysporum, Fusarium sambucinum, Rhizoctonia solani, Alternaria solani, Alternaria, tenuissima, Alternaria alternata, Colletotrichum coccodes, Phoma exigua, Pectobacterium carotovorum, and Streptomyces scabiei, by the agar-well diffusion method. Pulcherrimin formation, enzymatic profiles detected by the API ZYM system, and metabolite formation evaluated by HPLC analysis were conducted for the most active M. pulcherrima isolates. Leucine arylamidase, valine arylamidase, α- and β-glucosidase, and esterases were the most noteworthy in the pattern of activity. In turn, ethanol, glycerol, and organic acids (acetic, succinic, lactic acids) were determined in the largest quantities. The isolate M. pulcherrima TK1 was selected and cultured on supplemented acid whey. An in situ experiment was carried out on the seed potatoes, which showed a 30%–100% reduction in nine phytopathogens; only P. carotovorum was insensitive to yeast treatment. Therefore, M. pulcherrima TK1 was proposed as the potential biological solution for seed potato protection against phytopathogens.

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    Inhaltsbeschreibung

    Projekt

    Zdrowy Sadzeniak

    Zdrowy Sadzeniak

    Standort
    • Poland
    Verfasser*innen
    • Aleksandra Steglińska
    Zweck
    • Dissemination
    Dateiformat
    Document
    Dateigröße
    2.74 MB
    Erstellt am
    18-10-2022
    Originalsprache
    English
    Offizielle Projekt-Webseite
    Zdrowy Sadzeniak
    Lizenz
    CC BY
    Schlagworte
    • biopesticide
    • enzymatic activity
    • pulcherrimin
    • acid whey
    • antifungal activity

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